q5 high gc enhancer (New England Biolabs)

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New England Biolabs q5 high gc enhancer
Q5 High Gc Enhancer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/q5+high+gc+enhancer/Q5+Reaction+Buffer/pmc13076415-74-17-38
Average 99 stars, based on 1796 article reviews

q5 high gc enhancer - by Bioz Stars, 2026-09

99/100 stars

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Polymerase Chain Reaction:

Article Title: Co-transcriptional folding orchestrates sequential multi-effector sensing by a glycine tandem riboswitch
Article Snippet: .. Briefly, 14 μl of PCR master mix was added to 6 μl of a 16-fold dilution of the bead-bound cDNA libraries, such that the final concentration of components in the 20 μl PCR was: 1X Q5 Reaction Buffer (New England Biolabs), 1× Q5 High GC Enhancer (New England Biolabs), 200 μM dNTP Solution Mix, 250 nM RPIX Forward Primer (Table ), 250 nM dRP1_NoMod.R Reverse Primer (Table ), 10 nM RPIX_SC1_Bridge (Table ), and 0.02 U/μl Q5 High-Fidelity DNA Polymerase. ..

Article Title: Molecular basis for anti-jumbo phage immunity by AVAST type 5
Article Snippet: .. Phage genes were PCR-amplified using Q5 DNA polymerase (New England BioLabs) supplemented with Q5 High GC Enhancer (New England BioLabs) with primers (Integrated DNA Technologies) containing overhangs homologous to the pSTDesR plasmid. ..

Article Title: Co-occurrence networks reveal candidate AMF–microbe assemblages for generalist and crop-specific inocula
Article Snippet: PCR amplifications were performed from 1 ng of extracted DNA using a Surecycler 8800 thermal cycler (Agilent, Technologies, Les Ulis, France). .. The reaction mixtures (25 μL) contained 5 μL of 5× Q5 reaction buffer, 3 μL of 5× Q5 High GC enhancer (for fungal PCR), 0.25 μL (2 U μL − 1 ) of Q5 ® High-Fidelity DNA Polymerase (New England Biolabs France, Evry, France), 0.8 μL of each primer (0.4 μM), 1 μL of dNTPs (0.2 mM), 1 μL of DMSO, 1 μL of Bovine Serum Albumin (BSA; 100 μg/mL − 1 ) and 1 ng of DNA template. .. For bacterial 16 S rRNA PCR, thermocycling conditions were as follows: initial denaturation at 95 °C for 3 min, followed by 35 cycles of denaturation at 95 °C for 30 s, primer annealing at 56 °C for 30 s and extension at 72 °C for 50 s, with a final extension at 72 °C for 5 min. For fungal ITS region PCR, thermocycling conditions were as follows: initial denaturation at 95 °C for 10 min, followed by 35 cycles of denaturation at 94 °C for 20 s, primer annealing at 47 °C for 30 s and extension at 72 °C for 20 s, with a final extension at 72 °C for 5 min. All PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with GelRed ® (1:10,000; Biotium Inc., Fremont, CA, USA) at 60 V for 40 min and visualized using the Gel-Doc system (Bio-Rad Laboratories, Mississauga, ON, Canada).

Concentration Assay:

Mutagenesis:

Article Title: Molecular basis for anti-jumbo phage immunity by AVAST type 5
Article Snippet: .. Point mutations in the Sir2 or ATPase domains, as well as deletions within the sensor or putative signal peptide domains of Avs5 in pUCP20, were introduced using round-the-horn site-directed mutagenesis with 5′ phosphorylated primers (Integrated DNA Technologies) and Q5 DNA Polymerase supplemented with Q5 High GC Enhancer (New England BioLabs). .. The resulting PCR products were digested with DpnI (New England BioLabs), separated on 1% agarose gels, and the bands were excised and purified using the Zymo Gel DNA Recovery Kit.

Amplification:

Article Title: Molecular basis for anti-jumbo phage immunity by AVAST type 5
Article Snippet: .. The PaAvs5 systems and their promoter regions were amplified from Pseudomonas aeruginosa strains using the primers (Integrated DNA Technologies) listed in the and Q5 High-Fidelity DNA Polymerase (New England BioLabs) supplemented with Q5 High GC Enhancer (New England BioLabs). .. The resulting PCR products mixed with loading dye were visualized on 1% agarose gels supplemented with SYBR Safe DNA Gel Stain (Invitrogen), and the target bands were excised and purified with the Zymoclean Gel DNA Recovery Kit (Zymo Research).

other:

Article Title: Single-molecule DNA flow-stretch assays for high-throughput DNA-protein interaction studies.
Article Snippet: Q5 High GC Enhancer (#B9028A, New England Biolabs).

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q5 high gc enhancer (New England Biolabs)

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